Michal Pechar, PhD
TopicSynthetic hydrophilic polymers for siRNA delivery
AnnotationSynthesis, characterization and biological evaluation of polymer systems for delivery of small interfering RNA (siRNA) is proposed. The molecule of siRNA will be attached to a synthetic hydrohilic polymer carrier either via a covalent biodegradable bond enabling release of siRNA inside the target cells or non-covalently by means of cooperative ionic interactions between the phosphate groups of siRNA and cationic groups incorporated into the structure of the polymer carrier. The ionic interactions will lead to formation of polyelectrolyte complexes. Cell penetrating peptides facilitating transport of the macromolecules across the cell membrane will be also bound to the polymer carriers. The prepared polymer conjugates and polyplexes will be also actively targeted towards the desired cells using receptor-specific ligands based on synthetic oligopeptides or recombinant proteins, e.g. antibody fragments. The results of the project should significantly contribute to development of new siRNA-based macromolecular therapeutics against various types of disease.

Otto Wichterle Centre of Polymer Materials and Technologies - CPMTOW

Centre of Biomedicinal Polymers - CBMP

Centre of Polymer Sensors - CPS

Polymers for Power Engineering - Energolab


Institute of Macromolecular Chemistry AS CR
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162 06 Prague 6
Czech Republic
tel:+420 296 809 111
fax:+420 296 809 410

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