|Topic||Xeno-free enzymatically degradable polymer materials for 4D bioprinting|
|Supervisor||Vladimír Proks, Ph.D.|
|Consultant||MSc. Jana Dvořáková, Ph.D.|
|Department||Biomaterials and Bioanalogous Systems|
|Description||Current biology has opened new avenue in biotechnological R&D aimed at ex vivo building 3D structures that closely resemble tissues/organs of living organism. Despite of self-organizing capacity of cells, extracellular 3D support is still envisioned to promote establishment of proper tissue morphologies. 3D bioprinting is attractive option of how cells can be positioned into right locations and supported in their development. Advanced concept is so called 4D bioprinting,
defined by materials capable of post-printing responsiveness to stimuli. The key limitation to this approach lays in suboptimal chemistry of biomaterials, not providing enough flexibility in mechanical properties, internal geometry, ligand capture and release, etc.
The dissertation will focus on design, synthesis and study of physicochemical properties of polymeric precursors based on synthetic poly(amino acids)peptides. Furthermore, a 3D printing protocol will be developed to establish a hydrogel network, which could ensure mechanical protection of cells from shear forces and promote cell retention and engraftment. Hydrogels will be modified with biomimetic structures, e.g., cell-adhesion peptides that would promote specific interactions with cells and growth factors. Knowledge and skills in macromolecular and organic chemistry is expected as well as willingness to improve knowledge in biochemistry and biology. Student will learn various techniques and methods using modern measuring instruments.