| Description |
Bacterial infections, particularly those of the biofilm type, represent an increasing challenge for modern medicine, primarily due to rising antibiotic resistance. Cationic amphiphiles are highly effective local bactericides; however, for practical use on wounds, mucosal surfaces, or technical materials, it is often more advantageous to apply them not as concentrated solutions but in the form of controlled-release systems. Such formulations enable the long-term maintenance of lower, yet still bactericidal, concentrations of these agents, which are no longer harmful to human tissues. The aim of this doctoral dissertation is to prepare amphiphilic polyanions with varying structures and charge densities designed for the encapsulation and controlled release of micelles of cationic bactericides. The work will focus on elucidating the relationships between the structure of the polyanion and the bactericide, the efficiency of supramolecular encapsulation based on Coulombic interactions, the structure of the resulting polyplexes, and the release kinetics of the active component as influenced by temperature, ionic strength, and pH, as well as the associated bactericidal effects. A broad range of physicochemical methods will be employed to characterize these systems, including scattering techniques, fluorescence spectroscopy, isothermal titration calorimetry, and biological assays of antibacterial activity. |
