Supervisor
(Consultant)
Robert Pola, PhD
TopicTargeted polymeric drugs with enhanced penetration into tumor cells
AnnotationThe theme of the thesis will be focused on the development and study of active targeted polymeric drugs. Currently studied polymeric drug conjugates of synthetic polymer and biologically active substances (drugs) are very effective in treating variety of diseases, especially cancer. The aim of the work is to design structures, synthesis, physico-chemical characterization and verification of the biological activity of anticancer polymer drugs with improved specificity and efficiency. Increasing of the therapeutic efficacy of the polymeric drugs should be achieved by binding of specific ligands (prepared short peptide sequences) to the polymeric carrier bearing a drug. Selection of suitable oligopeptides and methods to attach them to the polymer in combination with the choice of active drug should lead not only to an increase in drug binding specificity to the target cell, but also to improve penetration of the polymeric drug to the cell and activate the drug inside the cell. This will also improve the therapeutic effect of the conjugate. Student will participate in selecting of suitable oligopeptides, their synthesis by solid phase, synthesis of monomers and polymeric carriers of different structure, the study of the binding reaction, physico-chemical characterization of the products (liquid chromatography, mass spectroscopy, light scattering), and in vitro biological studies properties of prepared conjugates (flow cytometric and fluorescence confocal microscopy) on various cell lines.

BIOpolymer POstdoctoral Laboratory and educational center - BIOPOL

Otto Wichterle Centre of Polymer Materials and Technologies - CPMTOW

Centre of Biomedicinal Polymers - CBMP

Centre of Polymer Sensors - CPS

Polymers for Power Engineering - Energolab


 

Institute of Macromolecular Chemistry AS CR, v.v.i.
Heyrovského nám. 2
CZ-162 06 Praha 6
Czech Republic
phone:+420 296 809 111
fax:+420 296 809 410

Strategie 21