Jan Dohnálek
Consultant Jarmila Dušková
Topic3D structure analysis of chitinolytic enzymes of bacterial microflora of the gastro-intestinal tract.
AnnotationBacteria inhabiting human gastro-intestinal tract express several enzymes capable of chitin cleavage. This complex of chitinolytic enzymes degrades chitin-containing cell walls of fungi and contributes to human health. A detailed study of enzymes of one of these bacteria provides important characteristics and properties of the molecules, including determination of their three-dimensional structure by the means of x-ray diffraction. The work is focused on enzyme isolation from bacterial extracts, their purification, activity assays,crystallization and determination of their three-dimensional structure by x-ray diffraction analysis followed by computational analysis of the obtained results.

Otto Wichterle Centre of Polymer Materials and Technologies - CPMTOW

Centre of Biomedicinal Polymers - CBMP

Centre of Polymer Sensors - CPS

Polymers for Power Engineering - Energolab


Institute of Macromolecular Chemistry AS CR
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