Department of Biological Models

Research People Equipment Publications

The department consists of four laboratories with fully established molecular biological methods and entirely equipped with apparatuses and instruments allowing the examination of the structure and functioning of gene expression and its regulation, structural and functional relations between information molecules and polymeric biomaterials. The laboratories are specially equipped for cultivation of a broad spectrum of cell lines (primary, transformed and immortalized), examination of  cell viability and cell line characteristics after interaction with biomaterials and observation of cell signalling and cell death identification using  flow cytometry, fluorescent or confocal microscopy. Evaluation of antimicrobial potential of polymeric biomaterials together with production and isolation of recombinant proteins is also a constituent part of the processes.

      The main objective of the Department is to establish flexible interdisciplinary cooperation during development of biomaterials, to offer results from first biological tests, which would contribute to modification, selection and improvement of properties of exerted materials.  Furthermore, the focus on developing new methodological approaches for studying the interaction of polymeric materials and cells. This new knowledge entails a broad spectrum of detailed studies and cooperation between experts from several fields.

Research

Research activities of the Department comprise following topics:

  1. Effect of polymer drug/diagnostic compound carriers on the cell behaviour 

We study the interaction of polymer therapeutics, especially polymer conjugates, with the cells in vitro. Polymers predominantly based on N-(2-hydroxypropyl)methacrylamide (HPMA) carry biological active compounds – especially antitumor drugs, diagnostic moieties or their combination usually bound to the polymer carrier via biodegradable spacers. The objective is to monitor their behaviour (especially cytotoxicity, cell uptake, penetration  into cells, intracellular transport and localization) in relation to the structure of the polymeric carrier, the content  of diagnostic or therapeutic agents the biodegradable spacer type and the presence of targeting molecules (peptide, antibody). We also  deal with polymer vaccines and polymeric systems for gene delivery.

  1. Effect of magnetic nanoparticles, nanocrystals on the cell behaviour

We study  the cytotoxicity of magnetic nanoparticles and their effect on cell proliferation, moreover, their intracellular localization with respect to their further application, e.g. for diagnostics.

  1. Evaluation of modified surfaces stimulate osteogenic differentiation of cells designed for tissue repair

We monitor  proliferation and differentiation characteristics of cells grown on modified surfaces based on polyethylene oxide (PEO)/PLA intended e.g. for the repair of bone tissue.  We further study the impact of carbone nanotubes for their  stimulation of cell differentiation.

  1. The nanofiber carriers intended for tissue repair and as depot of cytostatic drugs

We are focused on monitoring cytotoxicity of the nanofiber carriers mainly based on chitosan/gelatin/polylactic acid (PLA) using various cell lines. Furthermore, we study the growth and differentiation properties of primary cells grown on such carriers, e.g. retinal pigment epithelium cells, potentially usable for transplantation.

  1. Biological properties of the hydrogel systems suitable for the repair of the central nervous system (CNS) and other soft tissues

We observe the growth and differentiation characteristics of embryonic or mesenchymal stem cells on the hydrogel based surfaces with respect to their chemical and physical properties. The aim is to optimize hydrogels for in vivo applications and medical use.

  1. Microbial properties of Hemagel

We are focused on the study of antimicrobial properties of HemaGel and its modifications for medical applications.

BIOpolymer POstdoctoral Laboratory and educational center - BIOPOL

Otto Wichterle Centre of Polymer Materials and Technologies - CPMTOW

Centre of Biomedicinal Polymers - CBMP

Centre of Polymer Sensors - CPS

Polymers for Power Engineering - Energolab


 

Institute of Macromolecular Chemistry AS CR, v.v.i.
Heyrovského nám. 2
CZ-162 06 Praha 6
Czech Republic
phone:+420 296 809 111
fax:+420 296 809 410

Strategie 21