Laboratory of Polymer biomaterials for regenerative medicine

Research People Equipment Publications

 

(under BIOCEV program 4.1.3. Polymeric Biomaterials for Regenerative Medicine)

(head Mgr. Dana Kubies, CSc.)

The important factor supporting integration of biomaterials with the tissue of recipient in biomedical applications is immobilization of biologically active substances on the biomaterial surface. Our research activities are focused on development of technologies for surface modifications. The studied coating technologies are tools for controlled introduction of biological active substances (such as specific peptides or growth factors) on the surface of porous as well as nonporous implants. In this way we aim to modulate the interaction of the implant surface with the host tissue in the place of implantation (e.g. adsorption of proteins, adhesion, proliferation or differentiation of cells or surface endothelization). We also focus our activities on preparation of 3D macroporous polymer scaffolds for cell transplantation and for tissue regeneration and replacement applications.

 

Research areas

  • Surface modification of biomaterials for increase of their bioactivity
  • Preparation of 3D polymer scaffolds for tissue engineering applications

 

Research projects:

  • Deposition of colloid solutions of functionalized block copolymers for introduction of specific peptides and growth factors on polylactide surfaces
  • Deposition of polyelectrolyte multilayers prepared by a layer-by-layer deposition method with subsequent noncovalent immobilization of growth factors and preparation of polyelectrolyte particles loaded with growth factors
  • Preparation of polyelectrolyte particles for growth factor delivery
  • Deposition of organized fibrin nanostructures growing from the surface by a catalytic action of the surface-bound thrombin on the solution containing fibrinogen and subsequent covalent and non-covalent immobilization of growth factors. Surfaces of various biomaterials are coated by fibrin meshes of a thickness adjustable from 40 nm to several micrometers. Fibrin is grown by the catalytic action of thrombin immobilized on the surface to fibrinogen contained in an ambient fluid. Specific biological activities are added to the coating by the attachment of heparin, growth factors, antibodies, and extracellular matrix macromolecules, such as, fibronectin, laminin, collagen, or hyaluronic acid. The coatings have been applied for promoting endothelialization of blood vessel prostheses, colonization of artificial decellularized heart valves with selected cells, and for improving bloodcompatibilityof endovascular stents. Coatings, which are based on polymer brushes grafted from a biomaterial surfaces and subsequently modified with attached bioactive molecules, are being developed.

 

Co-operations:

  • Institute for Clinical and Experimental Medicine (IKEM),  Diabetes Center, Czech Republic · Praha (MUDr. Jan Kříž, PhD)
  • Dept. of Biomaterials and Tissue Engineering,  Institute of Physiology ASCR, Prague  (Doc. Lucie Bačáková, CSc.)
  • Institute of Haematology and Blood Transfusion, Prague (Prof. Ing.  J. E. Dyr, DrSc.)
  • Institute of Science & Technology in Medicine, Keele University Medical School, Stoke-on -Trent, UK (Prof. Alicia El Haj, Dr. Ying Yang)
  • Department for biomaterials research, Polymer Institute SAS, Bratislava, Slovakia (Ing. Igor Lacík, DrSc.)

BIOpolymer POstdoctoral Laboratory and educational center - BIOPOL

Otto Wichterle Centre of Polymer Materials and Technologies - CPMTOW

Centre of Biomedicinal Polymers - CBMP

Centre of Polymer Sensors - CPS

Polymers for Power Engineering - Energolab


 

Institute of Macromolecular Chemistry AS CR, v.v.i.
Heyrovského nám. 2
CZ-162 06 Praha 6
Czech Republic
phone:+420 296 809 111
fax:+420 296 809 410

Strategie 21