Supervisor
(Consultant)
Jan Dohnálek
Consultant Jarmila Dušková
Topic3D structure analysis of chitinolytic enzymes of bacterial microflora of the gastro-intestinal tract.
AnnotationBacteria inhabiting human gastro-intestinal tract express several enzymes capable of chitin cleavage. This complex of chitinolytic enzymes degrades chitin-containing cell walls of fungi and contributes to human health. A detailed study of enzymes of one of these bacteria provides important characteristics and properties of the molecules, including determination of their three-dimensional structure by the means of x-ray diffraction. The work is focused on enzyme isolation from bacterial extracts, their purification, activity assays,crystallization and determination of their three-dimensional structure by x-ray diffraction analysis followed by computational analysis of the obtained results.

Center BIOPOL

Center BIOpolymer POstdoctoral Laboratory and educational center arises with finantial support from EU funds, operational programme Education for Competitiveness


Institute of Macromolecular Chemistry AS CR, v.v.i.
Heyrovského nám. 2
CZ-162 06 Praha 6
Czech Republic
phone:+420 296 809 111
fax:+420 296 809 410